Collagen synthesis assay

Objectification of the synthesis of collagen from fibroblast culture medium
- Dosage de la synthèse de collagène
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Objectification of an anti-collagenase response

- Fibroblast culture
- Determination of the collagenase activity
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In vitro objectification of hydrating effect

by analysing the synthesis of glycosaminoglycans
- In vitro objectification of hydrating effect by analysing the synthesis of glycosaminoglycans
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Objectification of an anti-elastasic effect

- Evaluation of anti-elastasic activity in tubes
- Evaluation of anti-elastasic activity on frozen material
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Objectification of toxic outcome

- Toxicity of fibroblast or keratinocyte cultures
- Cellular coloration by Giemsa
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Objectification of a modulation in turn-over concerning epithelial cells

- Analysis of the proliferation of keratinocytes
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Objectification of an anti-radical effect

- Utilising fibroblastic and keratinocytal cultures :
A possibility to generate free radicals according to two systems
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Objectification of a healing effect by analyzing a proliferation of fibroblasts

- Fibroblastic culture :
Cell count after exclusion test using trypan blue

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