EXPERIMENTAL MODELS > In vitro reconstitution of skin equivalent [ DERMATOLOGY COSMETOLOGY ]
III. In vitro reconstitution of skin equivalent : METHOD
Histological and immunohistochemical analysis :
- of epithelial proliferation
- of epithelial differentiation
Example : evaluation of a product or ray meant to have healing properties
More info : Histological and immunohistochemical analysis
Immunohistochemical analysis of a neo-formed epithelium
- by mixing type 1 collagen with fibroblasts : formation of a lattice by collagen condensation in a sterilizer/drying oven,cupboard/incubator at 37°C.
More info : Implementation of a dermal equivalent
Epidermisation of equivalents by the implantation of a epidermis fragment
More info : Epidermisation of equivalents
Display of results
- - Photographs using optical and electron microscopes
- Establishment of semi-quantitative scores for cytokeratin immunochemistry
More infos : Display of results :
- IN VITRO RECONSTITUTION OF SKIN EQUIVALENT:
- Models :
- 1. Histological and immunohistochemical analysis :
- 2. Immunohistochemical analysis of a neo-formed epithelium
- 3. Epidermisation of equivalents by the implantation of a epidermis fragment
- 4. Display of results
- Human skin maintained in survival condition
- Cellular Cultures
- The in vitro reconstitution of skin equivalents
- Scalp sample maintained in survival conditions
- Clinical trial protocols (CCPPRB)
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