An experimental model
of human
skin
maintained in survival condition
Means of evaluation to test
the efficiency of
an active agent, a topical product, a nutritional supplement, or a device.
The in vitro reconstitution
of skin equivalents
Scalp sample maintained
in survival conditions
Clinical trial
protocols (CCPPRB)
GREDECO
DERMATOLOGY & COSMETOLOGY Research and Evaluation Group. GREDECO applies unique models of experimentation which are exclusively developed and standardized by its team
I. The evaluation of cosmetic and dermatologic products is carried out on human skin kept in a life-like state
Permits quick evaluation of reactions resulting from oral or topical application of a product or active ingredient, as well as those provoked by certain radiations (Lasers, Pulsed Light, LED, etc…) on different types of skin
Morphometric collagen analysis
Morphometric elastic fiber analysis
Analysis of vascular alteration
Turn-over activity of epithelial cells (differentiation, proliferation of basal cells)
Objectification ex vivo of the toxicity, penetration, or adherence of active ingredients on the skin
Study on human skin model’s tolerance (histology, cytokines, MTT test)
Collagen and elastin synthesis assay
Evaluation of the anti-elastasic effect of a product
Evaluation of the anti-collagenase activity of a product
Analysis of the in vitro synthesis of glycosaminoglycans
Free-radical scavenging activity (hydroperoxides assay..nitrite…)
II. Cellular Cultures : fibroblasts and keratinocytes
Collagen synthesis assay
Objectification of an anti-collagenase effect (metalloproteinases assay)
Analysis of cellular proliferation
Free-radical scavenging activity (hydroperoxides assay, analysis of cellular viability by MTT test after oxidative stress)
III. In vitro reconstitution of skin equivalent
Histological and immunohistological analysis of epithelial proliferation
Immunohistochemical analysis of epithelial differentiation
IV. Scalp sample maintained in survival conditions
Effectiveness of shampoo and topical products
Models of irritated, reactive, seborrheic scalp samples
V. Objectification resulting from skin prints visualized with a scanning microscope
Fine lines and wrinkles
Hydratation
Microdepressor network
VI. Clinical trial protocols (CCPPRB)
Therapeutic tests
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