peau humaine maintenue en survie par culture d'organe

01. Studying skin’s cellular tolerability
when cosmetics are administered

02. Analysis of an active agent’s penetration

03. Evidence of an anti-aging mechanism

04. Evaluation of a soothing product designed for over-reactive skin

05. Evaluation of solar protection

06. Evaluation of a depigmenting or pro pigmenting effect

07. Evaluation of anti-inflammatory effect

08. Objectification of elastin synthesis

09. Objectification of glycosaminoglycans synthesis

10. Evaluation of cellular desquamation

11. Study of cellular re-growth on an experimental model following superficial burns:

12. Anti-radical effect on an experimental model highlighting oxidative stress:

13. Evaluation of an anti-pollution effect (environmental or tobacco):

14. Evaluation of an anti-cellulite response:



I. Evaluation of cosmetic and dermatologic products carried out on human skin kept in a life-like state

GREDECO Exclusivity

  • anti age
  • microscope
  • produits cosmetiques

Our range of services specializing in dermato-cosmetic and stomatological clinical evaluations applies unique models of experimentation which are exclusively developed and standardized by Gredeco.

01. Tolerance test :

Studying skin’s cellular tolerability when cosmetics are administered

02. Analysis of an active agent’s penetration

03. Evidence of an anti-aging mechanism

- - Immunohistochemistry with anti-cytokeratin, anti-filaggrine, and anti-involucrate antibodies

- Two-dimensional electrophoresis gel used to demonstrate the presence of cytokeratins

- Establishment of semi-quantitative scores

- Immunohistochemistry with antibody anti-Ki67

- Count of positive cells brought to identical epithelial lengths

- Immunohistochemistry with anti-laminine antibodies, type IV anti-collagen

- Establishment of semi-quantitative scores

Two-dimensional electrophoresis gelTwo-dimensional electrophoresis gel

Two-dimensional electrophoresis gel allows for the identification of epithelial cytokeratines

Study based on anti-collagenStudy based on anti-collagen

Study based on anti-collagen basal membrane Ac, type IV

mmunohistochemical analysisImmunohistochemical analysis

Immunohistochemical analysis of the mitotic index on the epidermis with the help of antibody Ki67, which detects cells in a prolific phase

Immunohistochemical evaluationImmunohistochemical evaluation

Immunohistochemical evaluation of the final differentiation coming from a full anti-cytokeratin antibody

- Biochemical dose: after extraction by the pepsinum, the quantity of collagen is detected after special coloration made with sirius red

- Sircol Collagen Assay

- Histological quantification of elastic fibers through computer image analysis after coloration by (+) catechin

- % of occupied surface by elastic fibers, length (µm)

- Quantification of collagen through computer image analysis after coloration made with sirius red

- % of the surface occupied by the collagen

Mise en évidence histologique du collagèneIntact histological evidence of the collagen

Intact histological evidence of the collagen 5-(coloration effectuated with sirius red)

Fibres élastiques cutanéesElastic cellular fibers

Elastic cellular fibers coloured with (+) catechin

  • Evaluation of metalloproteinasic activity after IL1 or UV radiation stimulates the metalloproteinase

- Immunohistochemical proof (antibodies anti-MMP-1, MMP-2, MMP-9)

- Evaluation of collagenase activity of MMP1 in agarose gel containing type I collagen, measurement of the range of lysis

- Evaluation of gelatinase activity of MMP 2 & 9 on casein gel (zymogramme); measurement of the range of lysis

Evidence of an anti-aging mechanismEvidence of an anti-aging mechanism

Evaluation of metalloproteinasic activity

- Biochemical assay of glycosaminoglycans (kit Blyscan Glucosaminoglycan Assay)

- Histological evaluation of glycosaminoglycans after Hal coloration

- Immunohistochemical research of the hyaluronic acid receptor (CD44), trans-membrane glycoprotein

- Lipids assay via HPTLC

histological analysis of glycosaminoglycanshistological analysis of glycosaminoglycans

Objectification of cellular hydration by histological analysis of glycosaminoglycans (Hale coloration)

 

Update : december 2010



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