01. Studying skin’s cellular tolerability
when cosmetics are administered
02. Analysis of an active agent’s penetration
03. Evidence of an anti-aging mechanism
04. Evaluation of a soothing product designed for over-reactive skin
05. Evaluation of solar protection
06. Evaluation of a depigmenting or pro pigmenting effect
07. Evaluation of anti-inflammatory effect
08. Objectification of elastin synthesis
09. Objectification of glycosaminoglycans synthesis
10. Evaluation of cellular desquamation
11. Study of cellular re-growth on an experimental model following superficial burns:
12. Anti-radical effect on an experimental model highlighting oxidative stress:
13. Evaluation of an anti-pollution effect (environmental or tobacco):
14. Evaluation of an anti-cellulite response:
I. Evaluation of cosmetic and dermatologic products carried out on human skin kept in a life-like state
GREDECO Exclusivity
Our range of services specializing in dermato-cosmetic and stomatological clinical evaluations applies unique models of experimentation which are exclusively developed and standardized by Gredeco.
Studying skin’s cellular tolerability when cosmetics are administered
- - Immunohistochemistry with anti-cytokeratin, anti-filaggrine, and anti-involucrate antibodies
- Two-dimensional electrophoresis gel used to demonstrate the presence of cytokeratins
- Establishment of semi-quantitative scores
- Immunohistochemistry with antibody anti-Ki67
- Count of positive cells brought to identical epithelial lengths
- Immunohistochemistry with anti-laminine antibodies, type IV anti-collagen
- Establishment of semi-quantitative scores
Two-dimensional electrophoresis gel
Two-dimensional electrophoresis gel allows for the identification of epithelial cytokeratines
Study based on anti-collagen
Study based on anti-collagen basal membrane Ac, type IV
Immunohistochemical analysis
Immunohistochemical analysis of the mitotic index on the epidermis with the help of antibody Ki67, which detects cells in a prolific phase
Immunohistochemical evaluation
Immunohistochemical evaluation of the final differentiation coming from a full anti-cytokeratin antibody
- Biochemical dose: after extraction by the pepsinum, the quantity of collagen is detected after special coloration made with sirius red
- Sircol Collagen Assay
- Histological quantification of elastic fibers through computer image analysis after coloration by (+) catechin
- % of occupied surface by elastic fibers, length (µm)
- Quantification of collagen through computer image analysis after coloration made with sirius red
- % of the surface occupied by the collagen
Intact histological evidence of the collagen
Intact histological evidence of the collagen 5-(coloration effectuated with sirius red)
Elastic cellular fibers
Elastic cellular fibers coloured with (+) catechin
- Immunohistochemical proof (antibodies anti-MMP-1, MMP-2, MMP-9)
- Evaluation of collagenase activity of MMP1 in agarose gel containing type I collagen, measurement of the range of lysis
- Evaluation of gelatinase activity of MMP 2 & 9 on casein gel (zymogramme); measurement of the range of lysis
Evidence of an anti-aging mechanism
Evaluation of metalloproteinasic activity
- Biochemical assay of glycosaminoglycans (kit Blyscan Glucosaminoglycan Assay)
- Histological evaluation of glycosaminoglycans after Hal coloration
- Immunohistochemical research of the hyaluronic acid receptor (CD44), trans-membrane glycoprotein
- Lipids assay via HPTLC
histological analysis of glycosaminoglycans
Objectification of cellular hydration by histological analysis of glycosaminoglycans (Hale coloration)
Update : december 2010
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