peau humaine maintenue en survie par culture d'organe

01. Studying skin’s cellular tolerability
when cosmetics are administered

02. Analysis of an active agent’s penetration

03. Evidence of an anti-aging mechanism

04. Evaluation of a soothing product designed for over-reactive skin

05. Evaluation of solar protection

06. Evaluation of a depigmenting or pro pigmenting effect

07. Evaluation of anti-inflammatory effect

08. Objectification of elastin synthesis

09. Objectification of glycosaminoglycans synthesis

10. Evaluation of cellular desquamation

11. Study of cellular re-growth on an experimental model following superficial burns:

12. Anti-radical effect on an experimental model highlighting oxidative stress:

13. Evaluation of an anti-pollution effect (environmental or tobacco):

14. Evaluation of an anti-cellulite response:



I. Evaluation of cosmetic and dermatologic products carried out on human skin kept in a life-like state






04. Evaluation of a soothing product designed for over-reactive skin

Skin capillariesSkin capillaries (hemalun - eosine)

Histological visualisation of skin capillaries (hemalun - eosine)

  • Experimental model for over-reactive skin will be realized either:
  • - by epidermal irritation due to the application of lauryl sulphate
  • - establishment of semi-quantitative scores concerning the dermis and epidermis
  • - dose of cytokines
  • - by the application of a neuromediator (P substance)
  • - establishment of semi-quantitative scores for the evaluation of the oedema and dermal vasodilation.
  • - Cytokines assay

05. Evaluation of solar protection

Experimental model of radiation of the skin by UV A and B

solar protectionEvaluation of solar protection

  • Evaluation of UV B protection:

- Histology (quantification of sun-burnt cells and cellular alterations on the epidermis)

- Cellular viability (MTT test)

- Dosage of hydroperoxides in order to show evidence of anti-radical activity

- Dosage of nitrites

- Dosage of cytokines

  • Evaluation of UV A protection:

- - Histology of elastic fibers and of collagen (quantification analysis made from computer images)

- Dosage of cytokines.

06. Evaluation of a depigmenting or pro-pigmenting effect

Skin phototype III-IV maintained in survival for an evaluation of changes in depigmentation or conversely skin phototype II for the evaluation of propigmentation

depigmenting effectEvaluation of a depigmenting effect

Histologcial evaluation of the number of melanic pigments in the melanocytes and the adjacent keratinocytes (coloration by Fontana-Masson)

  • Evaluation of the melanin content after coloration with Fontana-Masson
  • Configuration of pigmentation scores
  • Evaluation of melanogenesis (DOPA reaction) by quantification of melanocyte count

 

07. Evaluation of anti-inflammatory effect

Experimental model of inflammation after the application of neuro-mediators or of irritants

anti-inflammatory effectEvaluation of anti-inflammatory effect

Histologcial evaluation of the number of melanic pigments in the melanocytes and the adjacent keratinocytes (coloration by Fontana-Masson)

  • Evaluation of an anti-inflammatory reaction :
    - Histology
    - Immunohistochemistry with the aid of antibodies anti-lymphocytes T, B
    - Cytokin assay

 

08. Objectification of elastin synthesis

elastin synthesis Objectification of elastin synthesis

- Remainder of skin cultures, kept in survival conditions

- Soluble elastin assay (Fastin Elastin Assay)

 

09. Objectification of glycosaminoglycans synthesis

glycosaminoglycans synthesisObjectification of glycosaminoglycans synthesis

- Extraction of glycosaminoglycans from the dermis by a papain

- Dose depending on Blyscan Glucosaminoglycan Assay.

 

 

Update : december 2010



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